ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of carbonic anhydrase (CA) IX and its significance in molecular subtyping of breast carcinomas. METHODL MaxVision immunohistochemical staining was used to examine the expression of ER, PR, HER2, CK5/6, EGFR, and CA IX in 117 cases of breast invasive ductal carcinomas.</p><p><b>RESULTS</b>The patients' age ranged from 25 to 71 years (mean 49.6 years). All the 117 cases were subclassified into five subtypes, with 66 (56.4%) luminal A, 6(5.1%) luminal B, 10 (8.6%) HER2 positive, 20 (17.1%) basal-like, and 15 (12.8%) unclassified tumors. The expression of CA IX in luminal A and basal-like breast cancers was 13.6% (9/66) and 8/20, respectively, with a significant difference (P < 0.05). Among the luminal A cancers, the expression of CA IX in tumors > 2 cm (7/27, 25.9%) was significantly (P < 0.05) higher than that of tumors ≤ 2 cm (2/39, 5.1%). The expression of CA IX in grade 3 invasive ductal carcinoma (18/50, 36.0%) was significantly higher than that in grade 1 (2/21, 9.5%) and 2 (7/46, 15.2%) tumors (both P = 0.006). In CA IX-negative of invasive ductal carcinoma, the expression of ER and PR was 61.1% (55/90) and 55.6% (50/90), respectively; whereas in CA IX-positive cancers, the expression of ER and PR was 37.0% (10/27) and 29.6% (8/27), respectively. The expression of hormone receptors in CA IX-negative tumors was significantly higher than that in CA IX-positive tumors (for both ER and PR, P < 0.05).</p><p><b>CONCLUSIONS</b>The expression of CA IX correlates not only with molecular subtypes of breast cancer, but also with the grading, hormone receptors and diameter of mammary invasive ductal carcinoma. CA IX is a relative independent marker of poor prognosis in breast cancer.</p>
Subject(s)
Adult , Aged , Female , Humans , Middle Aged , Antigens, Neoplasm , Metabolism , Biomarkers, Tumor , Metabolism , Breast Neoplasms , Classification , Metabolism , Pathology , Carbonic Anhydrase IX , Carbonic Anhydrases , Metabolism , Carcinoma, Ductal, Breast , Classification , Metabolism , Pathology , Neoplasm Grading , Receptor, ErbB-2 , Metabolism , Receptors, Estrogen , Metabolism , Receptors, Progesterone , Metabolism , Tumor BurdenABSTRACT
<p><b>AIM</b>To study the quantitative structure-activity relationship ( QSAR) of 23 tetrahydroimidazobenzodiazepinone (TIBO) as anti-HIV drug.</p><p><b>METHODS</b>A newly developed three-dimensional holographic vector of atomic interaction field (3D-HoVAIF) was used to describe the chemical structure of anti-HIV drug-23 TIBO, a partial least square regression (PLS) model was built.</p><p><b>RESULTS</b>The obtained model with the cumulative multiple correlation coefficient (Rcum(2)), cumulative cross-validated (Qcum(2)) and standard error of estimation (SD) were Rcum(2) = 0. 824, Qcum(2) = 0.778 and SD = 0.56, respectively. The model had favorable estimation stability and good prediction capabilities.</p><p><b>CONCLUSION</b>Satisfactory results showed that 3D-HoVAIF with definite physic-chemical meanings and easy structural interpretation for structural characterization could preferably express information related to biological activity of TIBO.</p>
Subject(s)
Algorithms , Anti-HIV Agents , Chemistry , Benzodiazepines , Chemistry , Holography , Methods , Imidazoles , Chemistry , Models, Molecular , Quantitative Structure-Activity RelationshipABSTRACT
Objective To explore the application and nursing of retention central vein catheter in hemodialy- sis.Methods Blood vessel path was established by inserted central vein catheter on 96 patients who needed hemodialysis treatment.Results Only one patient needed to tease tube for complication.Conclusion It's safe and effective to insert central vein catheter in hemodialysis.Applying and attending exactly can extend service life of catheter and prevent complication.